nanoparticles (NPs, 4.0 g/L) were applied for degradation of lignin from raw corn cob (CC) biomass for generation of cellulose-hemicellulose fractions amenable towards Clostridium cellulovorans during fermentation process. NP-treated biomass exhibited 43.26 % lignin removal from raw CC which was further employed for hydrogen fermentation by C. cellulovorans through CBP method. The strain yielded maximum 78.45 mL of cumulative hydrogen with hydrogen production rate of 1.55 mL/h using NP-treated CC. To the best of our knowledge, this is the first study on enhanced hydrogen production using NP-treated CC biomass in single pot fermentation which can prove to be a simpler, easier, and more economical process.