Tricin, a flavone belonging to the Gramineae family, has been confirmed to be the primary compound in a Zizania latifolia extract (ZLE) that prevents allergies.Various allergic reactions occur because of the unbalanced differentiation of T help cells (Th) and the consequent overproduction of IgE.Therefore, the regulation of Th1 and Th2 responses by T helper cell differentiation is essential for suppressing allergic responses.This study confirmed the immunomodulatory effects of ZLE and the major compound tricin in an OVA-sensitized mouse model.The IgE and OVA-specific production of tricin and ZLE in plasma were investigated in OVA-sensitized mice.The effects of www.videleaf.comtricin and ZLE on the amount of Th1 and Th2 cytokines and transcription factors released in splenocytes were investigated in OVA-sensitized mice.The skin roughness and the number of mast cells were confirmed by staining the skin surface with H&E and toluidine blue.Tricin and ZLE reduced the plasma IgE and OVA-specific-IgE levels significantly compared to the OVA group.On the other hand, tricin and ZLE promoted the release of the Th1 cytokines IL-12 and IFN- and inhibited the release of Th2 cytokines in OVA-sensitized mice.Tricin and ZLE induced T-bet and NFATc2 expression, anddown regulated GATA-3 levels.The skin roughness and the number of mast cells decreased in the OVA-immunized mice.Overall, the data indicate that tricin and ZLE may prevent allergy-related diseases through immunomodulation.