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gold
·인용수 0
·2025
Integrated biofabrication of artificial esophageal scaffolds using electrospinning, embedded DLP, and extrusion techniques
Palraj Ranganathan, Seung Hoon Han, In Gul Kim, Dageon Oh, Yewon Kim, Kyoung Ho Yoon, Garin Kim, Young Lee, Masoud Shirzad, Gyeongmi Lee, J. G. Choi, Jin Myoung Yoo, G.H. Lee, Young Jick Kim, Jungirl Seok, Ji Youl Lee, Sang‐Hyug Park, Jung‐Woog Shin, Seung Yun Nam, Eun‐Jae Chung
IF 10.2Materials Today Bio
초록

Esophageal reconstruction faces critical challenges due to limitations in current techniques, including inadequate mechanical properties, poor tissue integration, and insufficient functional regeneration. This study presents a novel biofabrication strategy for developing artificial esophageal scaffolds by integrating electrospinning, embedded digital light processing (DLP), and extrusion-based bioprinting techniques. These scaffolds are composed of flexible electrospun polyurethane (PU) nanofibers wherein silk fibroin methacryloyl (Sil-MA) is embedded within the PU layer to enhance mechanical strength and hydrophilicity. Decellularized esophageal extracellular matrix (EdECM) is deposited onto the scaffolds to promote tissue regeneration. Comprehensive in vitro and in vivo evaluations reveal that the PU/Sil-MA/EdECM scaffolds exhibit superior mechanical properties, enhanced cell adhesion, and significant improvements in smooth muscle and epithelial tissue regeneration. Moreover, in a rat model with partial esophageal defects, the scaffolds demonstrate successful tissue integration, reduced postoperative complications, and restoration of esophageal function, including peristalsis and nerve regeneration. Altogether, this integrated biofabrication approach offers a promising solution for esophageal reconstruction by effectively addressing the current challenges and paving the way for future clinical applications in regenerative medicine.

키워드
BiofabricationDecellularizationTissue engineeringFibroinExtracellular matrixMechanical strengthRegenerative medicineNanofiber
타입
article
IF / 인용수
10.2 / 0
게재 연도
2025