The medial prefrontal cortex (mPFC) and lateral habenula (LHb) plays key roles in drug addiction, decision-making, working memory, and other higher-order executive functions. Our previous studies have suggested that acupuncture at Shenmen (HT7) points modulates mesolimbic reward system in order to suppress drug-induced addiction behaviors. To explore whether a mPFC-LHb circuit mediates the inhibitory effects of acupuncture on addictive behaviors, we examined the projection from mPFC to LHb, excitation of mPFC neurons during acupuncture stimulation, the effects of optogenetic modulation of mPFC-LHb on HT7 inhibition of cocaine-induced locomotion and the effect of mPFC lesion on HT7 inhibition of NAc dopamine release.