Surface treatments play a crucial role in modifying the surface properties and biological performance of dental ceramics. This study investigated the effects of surface conditions on the wettability, cytocompatibility, and bacterial resistance of 4 mol% Y<sub>2</sub>O<sub>3</sub>-stabilized tetragonal zirconia polycrystal (4Y-TZP) and two lithium disilicate (Li<sub>2</sub>Si<sub>2</sub>O<sub>5</sub>) glass ceramics (Amber<sup>®</sup> Mill (AM) and Amber<sup>®</sup> Mill Abut-Crown (AC)). Human gingival fibroblast (HGF-1) responses and biofilm formation on the machined, polished, and glazed samples were evaluated. The polished 4Y-TZP sample exhibited the highest water contact angle (WCA; 71.3°), while that of the AC samples decreased as the sample was machined (58.4°), polished (46.8°), and glazed (14.0°). The wettability, cytocompatibility, and bacterial resistance of the dental ceramics were significantly influenced by material type and surface condition. Among the surface-treated samples, the glazed specimens exhibited the lowest WCA and bulk density; thus, wettability is an important factor for cell proliferation and bacterial resistance. Among all samples, HGF-1 cells adhered well to the glazed ceramics and significantly proliferated over time. Particularly, the 4Y-TZP and AC glazed samples exhibited the lowest biomass and strong resistance to biofilm formation and bacterial adhesion. Thus, the glaze dramatically affected HGF-1 cell growth and antibiofilm formation.