Nano/microparticles, being used in diverse fields including biotechnology and imaging, can be fabricated using various processes. Here, a functional surface with bio-polymer nano/microparticles and its fabrication method based on electrospraying are presented. The proposed nano/microparticulate surface can provide various beneficial effects on the cell culture behavior because of its morphological and geometrical properties. We experimentally show that the surface can provide the positive effects on cell attachment and culture with the comparison with the conventional flat surface.