Conducting polymers are often used as sensor electrodes due to their conjugated chain structure, which leads to high sensitivity and rapid response at room temperature. Numerous studies have been conducted on the structures of conducting polymer nanomaterials to increase the active surface area for the target materials. However, studies on the control of the chemical state of conducting polymer chains and the modification of the sensing signal transfer with these changes have not been reported. In this work, polypyrrole nanoparticles (PPyNPs), where is PPy is a conducting polymer, are applied as a sensor transducer to analyze the chemical sensing ability of the electrode. In particular, the protonation of PPy is adjusted by chemical methods to modify the transfer sensing signals with changes in the polymer chain structure. The PPyNPs that were modified at pH 1 exhibit high sensitivity to the target analyte (down to 1 ppb of NH3) with short response and recovery times of less than 20 s and 50 s, respectively, at 25 °C.