The human skin microbiome plays a crucial role in maintaining skin health by acting as a barrier against pathogens and modulating immune regulation. This case study investigates the skin microbiome of two healthy Korean male individuals in their 20s during Antarctic expeditions, focusing on microbial changes, reversion to pre-expedition states, and the influence of environmental and lifestyle factors. Notable microbial alterations were observed, including increases in Pseudomonadota and decreases in Actinomycetota, indicating pronounced microbial shifts in response to harsh environmental factors such as low temperature and humidity. Post-expedition revealed incomplete recovery to pre-expedition states, with Host A showing a higher resilience index, suggesting faster microbial recovery. Correlation analyses revealed associations between microbial changes and environmental factors (e.g., temperature, humidity, atmospheric pressure) as well as lifestyle factors (e.g., sunblock usage, outdoor activities), highlighting complex interactions between host behaviors and microbiome dynamics. Despite the study's limited sample size, these findings offer insights into the adaptability and resilience of the skin microbiome under extreme environments, with potential implications for health management and skincare strategies during isolated and prolonged expeditions.