Six human induced pluripotent stem cell (iPSC) lines (IPBi115-A, IPBi116-A, IPBi117-A, IPBi119-A, IPBi124-A, and IPBi129-A) were established from peripheral blood mononuclear cells (PBMCs) of healthy donors from Kazakhstan and Uzbekistan, representing Central Asian populations. These lines retained pluripotency, differentiated into the three germ layers, and showed normal karyotypes, with confirmed Sendai virus clearance, donor-matched STR profiles, and mycoplasma-free status. These iPSC resources expand population diversity for stem cell research and provide valuable platforms for disease modeling and drug screening, supporting human-based approaches in biomedical research.