Microorganisms from the genus <i>Hymenobacter</i> are known for their distinctive traits, yet their secondary metabolite (SM) production has not been thoroughly investigated. In this study, we examined the biosynthetic potential of SMs from <i>Hymenobacter psoromatis</i> PAMC26554, which was isolated from Antarctic lichen. In silico analysis identified biosynthetic gene clusters (BGCs) for terpenes, non-ribosomal peptide synthetases (NRPSs), and polyketide synthases (PKSs), indicating the strain's potential for novel SM production. Optimization of culture conditions showed that R2A medium at 15°C supported growth. HPLC analysis revealed phenylacetic acid (PAA) as a notable compound, which was characterized by ESI-MS and NMR, marking the first isolation of PAA from the genus <i>Hymenobacter</i>. In addition, bioactivity assays indicated moderate lipase inhibition by PAA, while molecular docking studies revealed stable interactions with the enzyme, demonstrating that hydrogen bonding and π-π stacking contribute to its lipase inhibitory activity. In summary, this research highlights the genus <i>Hymenobacter</i> as a potential source for secondary metabolite discovery, with PAA exemplifying its unexplored biochemical capabilities.