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·2025
High-resolution lymphography with multispectral photoacoustic microscopy
Thanh Dat Le, Yong-Jae Lee, Tae Joong Eom, Changho Lee
초록

Lymphography is an essential imaging technique for visualizing the lymphatic system, providing crucial insights into the morphological and functional information of lymphatic vasculature and nodes. This study introduces a state-of-the-art multispectral photoacoustic microscopy system (MPAM) specifically designed for high-resolution and high-speed photoacoustic lymphography that reveals the structure and functionality of lymphatic vascular and surrounded microvasculature. By leveraging the exceptional optical absorption contrast and depth imaging capabilities of photoacoustic microscopy (PAM), our system employs a wavelength-tunable Ti:sapphire pulsed laser in combination with a fixed primary laser at 532 nm. Our MPAM system operates at a 20 kHz repetition rate with a high-power conversion efficiency. The tunable Ti:sapphire laser operates from 740 nm to 840 nm, enabling real-time visualization and quantification of lymphatic vascular. With contrastive dye ICG by intradermal injection, we can present the micro-scale lymphatic system by using laser excitation at 790 nm. Our system allows single-scan visualization and quantification of micro-scale lymphatic system with a contrastive dye.

키워드
Multispectral imageMicroscopyPhotoacoustic imaging in biomedicineResolution (logic)Materials scienceOpticsRemote sensingGeologyComputer scienceArtificial intelligence
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2025