Photoacoustic microscopy (PAM) allows for the visualization of microscale structures and functionalities by detecting various chromophores in biological tissues based on their absorption spectra at specific laser wavelengths. PAM differentiates oxy and deoxy blood, water, collagen, and lipid with unique absorption spectra in NIR. To enhance the functionality of PAM, integrating multi-wavelength laser sources, particularly Ti:Sapphire lasers, is gaining significant attention. Ti:Sapphire lasers are considered an advanced solution due to their high energy efficiency and wavelength tunability. This presentation introduces a single-shot, wavelength-tunable Ti:Sapphire-based multispectral PAM system capable of rapidly performing functional imaging of blood concentrations and ICG-lymphography.