주요 논문
5
*2026년 기준 최근 6년 이내 논문에 한해 Impact Factor가 표기됩니다.
1
article
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인용수 8
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2025Generation of prostate cancer assembloids modeling the patient-specific tumor microenvironment
J.-H. Lee, Yun‐Hee Kim, Cheolmin Matthew Lee, Seong Soo Jeon, H.K. Seo, Jong Won Lee, Jungmin Choi, Minyong Kang, Eunjee Kim, Kunyoo Shin
IF 3.7 (2025)
PLoS Genetics
Prostate cancer (PC) is the most frequently diagnosed malignancy among men and contributes significantly to cancer-related mortality. While recent advances in in vitro PC modeling systems have been made, there remains a lack of robust preclinical models that faithfully recapitulate the genetic and phenotypic characteristics across various PC subtypes-from localized PC (LPC) to castration-resistant PC (CRPC)-along with associated stromal cells. Here, we established human PC assembloids from LPC and CRPC tissues by reconstituting tumor organoids with corresponding cancer-associated fibroblasts (CAFs), thereby incorporating aspects of the tumor microenvironment (TME). Established PC organoids exhibited high concordance in genomic landscape with parental tumors, and the tumor assembloids showed a higher degree of phenotypic similarity to parental tumors compared to tumor organoids without CAFs. PC assembloids displayed increased proliferation and reduced sensitivity to anti-cancer treatments, indicating that PC assembloids are potent tools for understanding PC biology, investigating the interaction between tumor and CAFs, and identifying personalized therapeutic targets.
https://doi.org/10.1371/journal.pgen.1011652
Tumor microenvironment
Prostate cancer
Biology
Organoid
Cancer
Stromal cell
Cancer research
Malignancy
Concordance
Cancer-Associated Fibroblasts
2
article
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인용수 2
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2025Single rosette-based generation of uniform cortical assembloids recapitulating cellular interactions between neurons and glial cells
E.H. Kim, Yunhee Kim, Soo-Jung Hong, I.S. Kim, J.-H. Lee, Jong-Yeon Yoo, Jihyun Kim, Kwangmin Yoo, Ho‐Jin Lee, Joung‐Hun Kim, Jungmin Choi, Kunyoo Shin
IF 15.7 (2025)
Nature Communications
Despite recent advances, current brain organoid technologies face ongoing challenges in managing heterogeneity and representing the diverse structure and cell types of the human brain. Here, we develop a module-based cellular reconstitution technology to sequentially build uniform cortical assembloids with mature cortical structures and functional connectivity. The uniformity and maturity of the cortical assembloids are achieved by creating single-rosette-based organoids at the early stage, whose sizes were big and consistent with the treatment of Wnt and Hedgehog agonists, followed by spatial reconstitution with the Reelin-expressing neuronal layer and non-neuronal glial cells. The resulting single-rosette-based cortical assembloids are highly uniform and reproducible without significant batch effects, solving major heterogeneity issues caused by difficulties in controlling the number and size of rosettes in conventional multi-rosette organoids. Furthermore, these cortical assembloids structurally and functionally recapitulate the physiology of the human brain, including the six-layered cortical structure, functional connectivity, and dynamic cellular interplay between neurons and glial cells. Our study thus provides an innovative preclinical model to study a range of neurological disorders, understanding the pathogenesis of which requires an organoid system capable of representing the dynamic cellular interactions and the maturity of the human brain.
https://doi.org/10.1038/s41467-025-66440-1
Organoid
Human brain
Cortical neurons
Cell type
Wnt signaling pathway
Hedgehog
Cellular neuroscience
3
article
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인용수 5
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2024Soo-Jung Hong, Ju Hee Lee, Yunhee Kim, E.H. Kim, Kunyoo Shin
IF 5.7 (2024)
Bioengineering & Translational Medicine
safe harbor locus-targeted, ChR2 knocked-in human pluripotent stem cells (hPSCs), followed by the differentiation of these genetically engineered hPSCs into forebrain organoids. The resulting ChR2-expressing human forebrain organoids showed homogeneous cellular expression of ChR2 throughout entire regions without any structural and functional perturbations and displayed consistent and robust neural activation upon light stimulation, allowing for the non-virus mediated, spatiotemporal optogenetic control of neural activities. Furthermore, in the hybrid platform in which brain organoids are connected with spinal cord organoids and skeletal muscle spheroids, ChR2 knocked-in forebrain organoids induced strong and consistent muscle contraction upon brain-specific optogenetic stimulation. Our study thus provides a novel, non-virus mediated, preclinical human organoid system for light-inducible, consistent control of neural activities to study neural circuits and dynamics in normal and disease-specific human brains as well as neural connections between brain and other peripheral tissues.
https://doi.org/10.1002/btm2.10690
Optogenetics
Organoid
Forebrain
Induced pluripotent stem cell
Neuroscience
Human brain
Biology
Cell biology
Locus coeruleus
Embryonic stem cell
4
review
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인용수 35
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2023A new era of stem cell and developmental biology: from blastoids to synthetic embryos and beyond
Yunhee Kim, I.S. Kim, Kunyoo Shin
IF 9.5 (2023)
Experimental & Molecular Medicine
Recent discoveries in stem cell and developmental biology have introduced a new era marked by the generation of in vitro models that recapitulate early mammalian development, providing unprecedented opportunities for extensive research in embryogenesis. Here, we present an overview of current techniques that model early mammalian embryogenesis, specifically noting models created from stem cells derived from two significant species: Homo sapiens, for its high relevance, and Mus musculus, a historically common and technically advanced model organism. We aim to provide a holistic understanding of these in vitro models by tracing the historical background of the progress made in stem cell biology and discussing the fundamental underlying principles. At each developmental stage, we present corresponding in vitro models that recapitulate the in vivo embryo and further discuss how these models may be used to model diseases. Through a discussion of these models as well as their potential applications and future challenges, we hope to demonstrate how these innovative advances in stem cell research may be further developed to actualize a model to be used in clinical practice.
https://doi.org/10.1038/s12276-023-01097-8
Biology
Stem cell
Stem cell biology
Model organism
Developmental biology
Organism
Computational biology
Relevance (law)
Model system
Embryo
5
article
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인용수 8
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2022Stem cells, organoids and their applications for human diseases: Special issue of BMB Reports in 2023
Kunyoo Shin
IF 3.8 (2022)
BMB Reports
Studying human biology has been challenging with conventional animal models or two-dimensional (2D) cultured cell lines. Recent advances in stem cell biology have made it possible to culture stem cells in vitro, leading to the establishment of in vitro three-dimensional (3D) organ-like structures known as organoids. Organoids are self-organizing 3D miniature tissues that mimic the tissue architecture and functionality of in vivo counterparts. Currently, organoids can be established for multiple tissues such as the intestine, brain, kidney, prostate, pancreas, liver, bladder, heart, and retina, either from pluripotent stem cells (PSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), or adult stem cells (AdSCs). In addition to normal organoids, patient-derived tumor organoids have been established from various human tumors such as pancreatic, colorectal, breast, liver, prostate, and bladder tumors. Also, bioengineering technologies including biomaterial and scaffold fabrication, bioprinting, and microfluidics have been recently applied to create more mature and complex organoids and miniature tissues in vitro. Incorporating recently advanced computational analyses including multi-omics profiling and bioinformatics further facilitated the process of using human organoids as a novel platform for human disease modeling, drug screening to identify potential targets and novel therapeutics, and the development of precision medicine and regenerative therapies. [BMB Reports 2023; 56(1): 1].
https://doi.org/10.5483/bmbrep.2022-0210
Organoid
Induced pluripotent stem cell
Stem cell
Embryonic stem cell
Biology
Regenerative medicine
Cell biology
Directed differentiation
Adult stem cell