Glioblastoma (GBM), the most aggressive malignant tumour, is increasingly treated with immunotherapy. The stimulator of interferon genes (STING) pathway is key to tumour immunity and a studied target for immunotherapy. This study explores the immune landscape of GBM, focusing on spatial relationships between tumour-associated immune cells (TAICs) and STING-expressing cells, uncovering patterns linked to prognosis. We studied 14 recurrent GBM patients using protein composition and Gene Ontology (GO) analysis and analyzed immune pathways in 69 newly diagnosed GBM patients undergoing standard therapy. Spatial analysis of cells was performed using QuPath, CytoMAP, and R. The spatial distribution of immune cells and their proximity to STING-expressing (STING+) cells are associated with survival in glioblastoma (GBM). Specifically, a closer distance between STING+ cells and CD4+ cells was linked to improved survival, while greater distances between STING+ cells and CD11c+ cells also correlate with better outcomes. This suggests that spatial relationships, not just the presence of cells, are important for prognosis.