Analyzing small RNA sequences from canine stem cell-derived extracellular vesicles primed with TNF-α and IFN-γ and exploring their potential in lung repair
Ji Sun Lee, Y.H. Jeong, Yohan Kim, Jang‐Hyuk Yun, Jin‐Ok Ahn, Jin Young Chung, Ju‐Hyun An
IF 2.9
Frontiers in Veterinary Science
Acute lung injury is an acute inflammation disorder that disrupts the lung endothelial and epithelial barriers. In this study, we investigated the extracellular vesicles (EVs) obtained via priming inflammatory cytokines such as tumor necrosis factor (TNF)-α and interferon (IFN)-γ on canine adipose mesenchymal stem cells in improving their anti-inflammatory and/or immunosuppressive potential, and/or their ability to alleviate lipopolysaccharide-induced lung injury <i>in vitro</i>. We also explored the correlation between epithelial-to-mesenchymal transition and the inflammatory repressive effect of primed EVs. Using small RNA-Seq, we confirmed that miR-16 and miR-502 significantly increased in EVs from TNF-α and IFN-γ-primed canine adipose mesenchymal stem cells. The pro and anti-inflammatory cytokines were analyzed in a lipopolysaccharide-induced lung injury model and we found that the EV anti-inflammatory effect improved on priming with inflammatory cytokines. EVs obtained from primed stem cells effectively suppress endothelial-to-mesenchymal transition in a lung injury model. Our results suggest a potential therapeutic approach utilizing EVs obtained from adipose mesenchymal stem cells primed with TNF-α and IFN-γ against lung inflammation and endothelial to mesenchymal transition.
IL-31RA and TRPV1 Expression in Atopic Dermatitis Induced with Trinitrochlorobenzene in Nc/Nga Mice
Seok‐Woo Lee, Na Yeon Lim, Min Soo Kang, Yunho Jeong, Jin‐Ok Ahn, Jung Hoon Choi, Jin Young Chung
IF 4.9
International Journal of Molecular Sciences
Atopic dermatitis (AD) is a common chronic inflammatory skin disease. Interleukin 31 (IL-31), a novel cytokine in AD, causes pruritus, typically characteristic of AD patients. The transient receptor potential vanilloid type 1 (TRPV1) is a cation channel activated by diverse noxious stimuli that has been studied in a variety of pruritic skin diseases. In this study, the AD animal model was generated by administering the hapten, trinitrochlorobenzene (TNCB), to Nc/Nga mice, and the degree of expression of the IL-31 receptor alpha (IL-31RA) and TRPV1 in the skin of these atopic models was evaluated. The Nc/Nga mice were divided into 3 groups: control, TNCB 2-weeks treated, and TNCB 8-weeks treated. After inducing AD, the skin lesions in each group were scored and compared, and the histology of the skin lesions and the IL-31RA and TRPV1 expression for each group were evaluated by analyzing immunohistochemistry. The results show a significant difference in the skin lesion scores between the groups. The immunohistochemistry evaluation highlighted the remarkable expression of IL-31RA and TRPV1 in the nerve fibers of the TNCB 8-weeks-treated group. We thus confirmed that the long-term application of TNCB induced chronic atopic-like dermatitis and that IL-31RA and TRPV1 were overexpressed in the peripheral nerve fibers in this AD model.
Induction of Osteogenesis After BMP-2 Injection in Rabbit Shoulders: A Novel Strategy for Managing Glenoid Bone Defect Associated with Anterior Shoulder Instability
Sae Hoon Kim, Dong Mo Kang, Jin Young Chung, Jung Hoon Choi, Sae Hoon Kim, Sang‐Yun Oh, Young Hoon Jang
IF 2.5
World Journal of Surgery
Background: Bone morphogenic protein-2 (BMP-2) has been shown to promote bone formation. The purpose of this research was to determine if an injectable form of BMP-2 could induce bone formation in anteroinferior glenoid bone defects, which is a critical factor of anterior shoulder instability. The purpose of this study was to investigate bone formation in rabbit shoulders following BMP-2 injection into the anterior capsulolabral complex. We hypothesized that the amount of new bone would be greater in rabbit shoulders injected with BMP-2 than in those injected with normal saline. Methods: An experimental study was conducted on 40 shoulders of 20 mature rabbits. Twenty right shoulders were allocated to four groups according to injection dose (5 µg or 10 µg) and follow-up period after injection (4 or 8 weeks). Twenty left shoulders were injected with normal saline as controls. At 4 or 8 weeks after injection, all rabbits were sacrificed, and bone volumes, densities, and locations of bone formation were evaluated using micro-computed tomography (micro-CT). Histologic analysis was performed on all 40 shoulders. Results: Shoulders injected with BMP-2 showed significantly more bone formation than control shoulders in all treated groups (58.6 ± 60.5 mm3 vs. 25.6 ± 32.6 mm3, p = 0.001). Moreover, bone formation increased with dose and follow-up. Locations of bone formation were the lesser tuberosity, greater tuberosity, and anterior capsulolabral complex. Histologic analysis confirmed the micro-CT-detected bone formation by revealing the presence of activated osteocytes, abundant osteoblastic rimming, and woven bone matrix. Conclusion: The study confirms that BMP-2 injection into the anterior capsulolabral complex of rabbit shoulders promotes new bone formation in adjacent soft tissue areas. Study Design: Controlled laboratory study.
Analyzing small RNA sequences from canine stem cell-derived extracellular vesicles primed with TNF-α and IFN-γ and exploring their potential in lung repair
Ji Sun Lee, Y.H. Jeong, Yohan Kim, Jang‐Hyuk Yun, Jin‐Ok Ahn, Jin Young Chung, Ju‐Hyun An
IF 2.9
Frontiers in Veterinary Science
Acute lung injury is an acute inflammation disorder that disrupts the lung endothelial and epithelial barriers. In this study, we investigated the extracellular vesicles (EVs) obtained via priming inflammatory cytokines such as tumor necrosis factor (TNF)-α and interferon (IFN)-γ on canine adipose mesenchymal stem cells in improving their anti-inflammatory and/or immunosuppressive potential, and/or their ability to alleviate lipopolysaccharide-induced lung injury <i>in vitro</i>. We also explored the correlation between epithelial-to-mesenchymal transition and the inflammatory repressive effect of primed EVs. Using small RNA-Seq, we confirmed that miR-16 and miR-502 significantly increased in EVs from TNF-α and IFN-γ-primed canine adipose mesenchymal stem cells. The pro and anti-inflammatory cytokines were analyzed in a lipopolysaccharide-induced lung injury model and we found that the EV anti-inflammatory effect improved on priming with inflammatory cytokines. EVs obtained from primed stem cells effectively suppress endothelial-to-mesenchymal transition in a lung injury model. Our results suggest a potential therapeutic approach utilizing EVs obtained from adipose mesenchymal stem cells primed with TNF-α and IFN-γ against lung inflammation and endothelial to mesenchymal transition.
IL-31RA and TRPV1 Expression in Atopic Dermatitis Induced with Trinitrochlorobenzene in Nc/Nga Mice
Seok‐Woo Lee, Na Yeon Lim, Min Soo Kang, Yunho Jeong, Jin‐Ok Ahn, Jung Hoon Choi, Jin Young Chung
IF 4.9
International Journal of Molecular Sciences
Atopic dermatitis (AD) is a common chronic inflammatory skin disease. Interleukin 31 (IL-31), a novel cytokine in AD, causes pruritus, typically characteristic of AD patients. The transient receptor potential vanilloid type 1 (TRPV1) is a cation channel activated by diverse noxious stimuli that has been studied in a variety of pruritic skin diseases. In this study, the AD animal model was generated by administering the hapten, trinitrochlorobenzene (TNCB), to Nc/Nga mice, and the degree of expression of the IL-31 receptor alpha (IL-31RA) and TRPV1 in the skin of these atopic models was evaluated. The Nc/Nga mice were divided into 3 groups: control, TNCB 2-weeks treated, and TNCB 8-weeks treated. After inducing AD, the skin lesions in each group were scored and compared, and the histology of the skin lesions and the IL-31RA and TRPV1 expression for each group were evaluated by analyzing immunohistochemistry. The results show a significant difference in the skin lesion scores between the groups. The immunohistochemistry evaluation highlighted the remarkable expression of IL-31RA and TRPV1 in the nerve fibers of the TNCB 8-weeks-treated group. We thus confirmed that the long-term application of TNCB induced chronic atopic-like dermatitis and that IL-31RA and TRPV1 were overexpressed in the peripheral nerve fibers in this AD model.
Induction of Osteogenesis After BMP-2 Injection in Rabbit Shoulders: A Novel Strategy for Managing Glenoid Bone Defect Associated with Anterior Shoulder Instability
Sae Hoon Kim, Dong Mo Kang, Jin Young Chung, Jung Hoon Choi, Sae Hoon Kim, Sang‐Yun Oh, Young Hoon Jang
IF 2.5
World Journal of Surgery
Background: Bone morphogenic protein-2 (BMP-2) has been shown to promote bone formation. The purpose of this research was to determine if an injectable form of BMP-2 could induce bone formation in anteroinferior glenoid bone defects, which is a critical factor of anterior shoulder instability. The purpose of this study was to investigate bone formation in rabbit shoulders following BMP-2 injection into the anterior capsulolabral complex. We hypothesized that the amount of new bone would be greater in rabbit shoulders injected with BMP-2 than in those injected with normal saline. Methods: An experimental study was conducted on 40 shoulders of 20 mature rabbits. Twenty right shoulders were allocated to four groups according to injection dose (5 µg or 10 µg) and follow-up period after injection (4 or 8 weeks). Twenty left shoulders were injected with normal saline as controls. At 4 or 8 weeks after injection, all rabbits were sacrificed, and bone volumes, densities, and locations of bone formation were evaluated using micro-computed tomography (micro-CT). Histologic analysis was performed on all 40 shoulders. Results: Shoulders injected with BMP-2 showed significantly more bone formation than control shoulders in all treated groups (58.6 ± 60.5 mm3 vs. 25.6 ± 32.6 mm3, p = 0.001). Moreover, bone formation increased with dose and follow-up. Locations of bone formation were the lesser tuberosity, greater tuberosity, and anterior capsulolabral complex. Histologic analysis confirmed the micro-CT-detected bone formation by revealing the presence of activated osteocytes, abundant osteoblastic rimming, and woven bone matrix. Conclusion: The study confirms that BMP-2 injection into the anterior capsulolabral complex of rabbit shoulders promotes new bone formation in adjacent soft tissue areas. Study Design: Controlled laboratory study.
Relationship Between Red Blood Cell Indices and Myxomatous Mitral Valve Disease in Small‐Breed Dogs: A Retrospective Study
Enrique Hong, Yunho Jeong, Ju‐Hyun An, Sooyoung Choi, Jin Young Chung, Jin‐Ok Ahn
IF 1.7
Veterinary Medicine and Science
We observed no correlation between RBC indices and MMVD. However, conflicting results have been reported in several other studies; thus, further studies should be considered.
The role of HbA1c in identifying dogs at high risk for diabetes despite normal blood glucose levels.
Jeong-Ho Ha, Yunho Jeong, Jin‐Ok Ahn, Jin Young Chung
PubMed
The objective of this study was to confirm the clinical utility of hemoglobin A1c (HbA1c) in identifying dogs at a high risk for diabetes. In this cross-sectional study, all the dogs were categorized into a control group and 3 experimental groups (overweight, elderly, and dogs with diseases that can contribute to diabetes). The control group included young and healthy dogs that visited veterinary clinics mainly for health checkups or neutering. The HbA1c concentration of all the dogs was estimated and compared between the control group and the experimental groups. The HbA1c level was significantly higher in the experimental groups than in the control group. In addition, a significant correlation was observed between the HbA1c concentration and all experimental variables. The HbA1c concentration was higher in the overweight group [mean, median: 3.94, 3.9; range (R): 3.5 to 4.3%], the elderly group [mean, median: 3.94, 3.9 (R: 3.5 to 4.3%)], and the disease group [mean, median: 3.99, 4.0 (R: 3.5 to 4.9%)] than in the control group [mean, median: 3.52, 3.6 (R: 2.9 to 3.8%)]. According to the results of this study, HbA1c can be used to evaluate high-risk groups for diabetes in the veterinary field, as in human medicine.
Quality Characteristics of Macaron as a Function of the Proportion of Gellan Gum in the Mixture of Egg White Substitute
Jin Young Chung, Lana Chung
Culinary Science & Hospitality Research
The objective of this study was to investigate the quality characteristics of macaron samples made from egg white substitutes with three different mixtures of gellan gum (GG), soy protein isolate (SPI), and maltodextrin (MD) and to find the ideal ratio of GG to SPI for macarons. For this study, a sample prepared with egg white as a control group, and four samples containing varying concentrations (1%, 2.5%, 5%, and 7.5%) of GG to SPI were prepared except for the control group. The analysis of pH, moisture value, °Brix, color, texture profiles, and sensory evaluation were conducted. Within the samples with GG-SPI-MD, darkness, and yellowness tended to decrease with increasing GG content up to GG5 (5% GG to SPI). Also the moisture content increased with increasing GG content. The control had the highest pH and °Brix value were significantly different from the sample made with the egg substitute. According to the texture profile analysis, hardness and fracture increased as the GG percentage increased until GG5. GG2.5 (2.5% GG to SPI) scored the highest in taste, texture, and overall acceptance in the consumer acceptance test. In conclusion, GG2.5 showed the most suitable quality characteristics for making macarons by mimicking egg white. Therefore, this study can be used as a basic research material for developing egg white substitutes using the component blend of GG-SPI-MD. However, it is judged that further research is needed to develop more viable applications.
Developing veterinary basic clinical skill items based on Korean Veterinary Entrustable Professional Activity
Kichang Lee, Heungshik S. Lee, Seong Mok Jeong, Jongil Kang, Seung-Joon Kim, Jin Young Chung, Sang‐Soep Nahm, Pan Dong Ryu
IF 1.5
Journal of Veterinary Science
The veterinary basic clinical skills established through this study can be used as a specific guide for clinical education in Korean veterinary school, and is expected to play an important role in meeting the needs of the educational sector of the veterinary education accreditation standards.
Presumed solitary ocular lymphoma of large B-cell origin with Mott cell change in a dog.
Yunho Jeong, Mark Chalkley, Ho‐Hyun Kwak, Sooyoung Choi, Jin Young Chung, Heung‐Myong Woo, Jin‐Ok Ahn
PubMed
A 4-year-old female Maltese dog was referred to our veterinary hospital with uveitis and conjunctivitis of the right eye. An ophthalmological evaluation revealed an intraocular mass that appeared to originate from the anterior uvea. Metastasis and regional invasion were not detected with CT examination. Enucleation of the right eye was recommended; however, the owner declined treatment. Six months later, the dog was re-presented with a right facial mass. At presentation, superficial lymph node enlargement was not appreciated, and no apparent alterations were noted on blood analysis or urinalysis. Computed tomography revealed an intraocular mass that invaded the surrounding tissues, including the frontal sinus. Presumed solitary ocular lymphoma with a large B-cell phenotype and Mott cell change was diagnosed <i>via</i> histopathological and immunohistochemical examination of a biopsy of the lesion. As the mass was too large for complete excision, neoadjuvant chemotherapy was administered. Complete remission was achieved using the L-COAP protocol and successful exenteration of the right eye. However, the dog was returned with enlargement of the right retropharyngeal lymph nodes. To the best of our knowledge, this is the first case report of presumed solitary ocular lymphoma with a large B-cell phenotype displaying Mott cell change in a dog. Key clinical message: This is the first reported case of a presumed solitary ocular lymphoma with a large B-cell phenotype and Mott cell change. Although systemic involvement was observed 6 mo after the initial visit, neoadjuvant chemotherapy and exenteration were effective.
Computed tomographic evaluation of portal vein indices in cats with the extrahepatic portosystemic shunts
Eunji Jeong, Jin Young Chung, Jin‐Ok Ahn, Hojung Choi, Youngwon Lee, Kija Lee, Sooyoung Choi
IF 1.5
Journal of Veterinary Science
The results demonstrated significant differences in PV indices between dogs and cats. In cats, the PV/Ao ratio demonstrated high diagnostic performance for symptomatic EHPSS. The PV diameter also performed well, in contrast to dogs.
