김기광 연구실
충남대학교 생화학과
김기광 교수
RNA 생물학
선택적 RNA 스플라이싱
암 정밀의료
홈
김기광 연구실
충남대학교 생화학과 김기광 교수
김기광 연구실은 RNA 대사와 세포 신호전달이 질병 및 세포 기능에 미치는 영향을 생화학·분자생물학적으로 분석합니다. 선택적 RNA 스플라이싱과 RNA 결합단백질의 조절 기전을 기반으로 PBRM1 등 암 관련 유전자의 아이소폼 변화, 면역관문 및 PARP 억제제 반응성을 규명하고 splicing-switch oligonucleotide와 multiplex qPCR 기반 진단·치료 기술을 개발합니다. 또한 CRISPR/Cas9으로 G3BP1 형광 리포터를 인간 폐 세포와 줄기세포 유래 오가노이드에 도입하여 스트레스 과립과 생체분자 응축체를 실시간 분석하고 미세먼지·화학물질의 인체 관련 독성을 평가합니다. cGAS-STING 및 NFAT 신호 조절을 활용한 NK 세포 항암활성, 근육세포 분화, 세포 배양육 응용 연구도 수행합니다.
RNA 생물학선택적 RNA 스플라이싱암 정밀의료생체분자 응축체인간 오가노이드
대표 연구 분야
연구 영역 전체보기
선택적 RNA 스플라이싱 기반 암 발생 및 치료 반응 조절 연구
Alternative RNA Splicing in Cancer Development and Therapeutic Response
연구 분야 상세보기
생체분자 응축체 및 인간 오가노이드 기반 독성 평가 연구
Biomolecular Condensates and Human Organoid-Based Toxicity Assessment
연구 분야 상세보기
세포 신호전달 기반 면역·근육 기능 조절 및 바이오소재 응용 연구
Cell Signaling-Based Regulation of Immune and Muscle Functions with Biotechnological Applications
연구 분야 상세보기
연구 성과 추이
표시된 성과는 수집된 데이터 기준으로 산출되며, 일부 차이가 있을 수 있습니다.
주요 논문
5
논문 전체보기
1
Article
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인용수 1
·
2026CRISPR-engineered human lung organoids with a biomolecular condensate reporter enable mechanistic toxicity monitoring
Seung-Yeon Kim, Ji-Won Baek, E. Jin Kim, E. Jin Kim, Sathiyaraj Srinivasan, Kee K. Kim, E. Jin Kim, E. Jin Kim
IF 10.2 (2026)
Materials Today Bio
Understanding how chemical stress perturbs human lung physiology requires models that capture dynamic molecular responses in real time. Here, we established a CRISPR/Cas9-engineered human induced pluripotent stem cell (hiPSC)-derived lung organoid expressing endogenous G3BP1–mCherry, enabling live, non-destructive visualization of stress granule (SG) formation under toxicant exposure. The organoids recapitulated airway and alveolar epithelial diversity and displayed lamellar body-like ultrastructures, indicating advanced maturation. Time-lapse imaging revealed rapid and reversible SG dynamics across chemically distinct stressors, while cytotoxicity assays showed that these organoids are significantly more sensitive than conventional 2D or cancer-derived lung models. Importantly, SG dynamics were linked to exposure duration–dependent changes in epithelial barrier integrity, indicating that SG formation precedes overt epithelial injury and serves as an early indicator of toxicant-induced cellular stress. Integration with high-content screening enabled quantitative, image-based analysis of cellular stress phenotypes, greatly enhancing throughput and mechanistic insight, thereby provided next-generation New Approach Methodologies for lung toxicity assessment. Together, this hiPSC-derived lung organoid SG reporter platform links early molecular stress adaptation to tissue-level responses, offering a predictive and mechanistically informative framework for human-relevant lung toxicity evaluation. ● CRISPR-engineered human lung organoids express endogenous G3BP1–mCherry reporter ● Live imaging enables rapid and reversible visualization of stress granules ● Stress granule dynamics act as an early and sensitive biomarkers of chemical stress ● Lung organoids show higher chemical sensitivity than immortalized and cancer cell models ● Reporter enables non-destructive, real-time monitoring of lung toxicant responses
https://doi.org/10.1016/j.mtbio.2026.102972
Organoid
Toxicant
Lung
Induced pluripotent stem cell
Toxicity
Cytotoxicity
Cell
2
Article
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인용수 25
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2025Biomolecular condensates: molecular structure, biological functions, diseases, and therapeutic targets
Sunkyung Choi, Jung‐Min Lee, Kee K. Kim
IF 13 (2025)
Molecular Biomedicine
Cells constantly encounter environmental and physiological fluctuations that challenge homeostasis and threaten viability. In response to these cues, specific proteins and nucleic acids engage in multivalent interactions and undergo phase separation to form membraneless assemblies known as biomolecular condensates. Nuclear condensates include paraspeckles, nuclear speckles, and Cajal bodies, while cytoplasmic condensates include stress granules, processing bodies, RNA transport granules, U-bodies, and Balbiani bodies. These assemblies regulate transcription, splicing fidelity, RNA stability, translational reprogramming, and integration of signaling pathways, thereby serving as dynamic platforms for metabolic regulation and physiological adaptation. However, dysregulation of these condensates has been increasingly recognized as a central pathogenic mechanism in neurodegenerative diseases, cancers, and viral infections, contributing to toxic protein aggregation, nucleic acid dysregulation, and aberrant cell survival signaling. This review provides a comprehensive synthesis of the molecular mechanisms governing condensation, delineates the diverse types and functions of major biomolecular condensates, and examines therapeutic approaches based on their pathophysiological relevance to disease development and progression. Furthermore, we highlight the cutting-edge technologies, including CRISPR/Cas-based imaging, optogenetic manipulation, and AI-driven phase separation prediction tools, which enable the real-time monitoring and precision targeting of cytoplasmic biomolecular condensates. These insights underscore the emerging potential of biomolecular condensates as both biomarkers and therapeutic targets, paving the way for precision medicine approaches in condensate-associated diseases.
https://doi.org/10.1186/s43556-025-00350-y
RNA
RNA splicing
Nucleic acid
Mechanism (biology)
Cytoplasm
Optogenetics
Signal transduction
Stress granule
3
Article
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인용수 2
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2025Enhancement of NK cell activity via DNA-sensing inhibition by Poxin transgene
Ling-Zu Kong, Seok‐min Kim, Soo Yun Lee, In-Hwan Jang, Chunli Wang, Seona Jo, Se‐Chan Oh, Sun‐Young Lee, Ji Hyun Kim, Kee K. Kim, Tae‐Don Kim
IF 13 (2025)
Journal of Advanced Research
INTRODUCTION: Natural Killer (NK) cell therapy has shown strong potential for cancer treatment; however, NK cell efficacy is limited by their short lifespan and suppression within the tumor microenvironment. One factor contributing to this suppression may be the activation of the cyclic GMP-AMP synthase (cGAS) pathway. Since the discovery of cGAS as a DNA sensor, there has been renewed interest in DNA sensing mechanisms, although the role of DNA sensing-mediated innate immune responses on NK cells remains unclear. Poxin, a protein derived from DNA viruses, is known to degrade the cGAS signaling product cyclic GMP-AMP (cGAMP) and could potentially alleviate DNA sensing-related stress in NK cells. OBJECTIVES: This study aims to investigate whether Poxin expression can inhibit DNA sensing pathways in NK cells, thereby enhancing their cytotoxic function and anti-tumor activity. METHODS: We generated NK-92 cells expressing a Poxin transgene and evaluated their response to DNA sensing activation. The cGAS-STING pathway was stimulated through either exogenous plasmid transfection or irradiation to induce endogenous double-stranded DNA (dsDNA). We assessed NK-92 and umbilical cord blood derived primary NK (pNK) cells activity through cytotoxicity assay and analysis of perforin and granzyme B expression levels, performed RNA-seq analysis to explore the mechanism and conducted functional assays to evaluate anti-tumor efficacy of NK-92 and chimeric antigen receptors (CAR)-NK-92 cells. RESULTS: Poxin expression significantly inhibited the cGAS-STING pathway, reducing activation in response to both plasmid transfection and irradiation-induced dsDNA. RNA-seq analysis indicated increased levels of cytotoxic mediators, including perforin and granzyme B, in Poxin-expressing NK-92 cells. Furthermore, these transgenic NK-92/CAR-NK-92 cells exhibited enhanced anti-tumor activity compared to controls. CONCLUSION: Poxin effectively suppresses DNA sensing-mediated innate immunity in NK cells, enhancing their cytotoxicity and anti-tumor effectiveness.
https://doi.org/10.1016/j.jare.2025.05.058
Transgene
DNA
Cell
Chemistry
Computer science
Gene
Biochemistry
최신 정부 과제
8
과제 전체보기
1
2025년 4월-2026년 12월
|140,000,000원선택적 스플라이싱 분석 기술의 항암제 반응성 예측용 진단키트 사업화 추진을 위한 IP 고도화 및 상용화
- 본 연구는 선택적 스플라이싱 (Alternative splicing) 분석 기반 항암제 반응성 예측 기술을 개발하고 이를 정밀 의료 진단 시스템으로 고도화 및 상용화하는 것을 최종 목표로 함. 기존 면역관문억제제 및 PARP 억제제의 치료 효과가 환자마다 다르게 나타나는 문제를 해결하기 위해 PBRM1 유전자 발현의 선택적 스플라이싱 패턴을 정량적으로 ...
암진단
선택적 스플라이싱
면역관문억제제
PARP 억제제
항암제
2
2025년 3월-2028년 2월
|474,056,000원크로마틴 재편성 SWI/SNF 복합체 구성유전자의 선택적 스플라이싱 조절을 통한 암 발생 기전 연구
기존 연구과제 수행을 통해 크로마틴 재편성 (Chromatin remodeling) SWI/SNF 복합체의 구성유전자 PBRM1의 암 특이적 선택적 스플라이싱 변화에 의한 암 유도 기전을 최초로 규명하였으며 이를 조절하는 획기적인 암 억제 후보물질을 성공적으로 개발하였음. 뿐만 아니라 PBRM1 외에도 다양한 SWI/SNF 복합체 구성유전자의 암 특이적인 ...
선택적 스플라이싱
크로마틴 재편성
암
후성유전학
RNA 치료제
3
2024년 8월-2027년 4월
|1,375,000,000원RNA 대사 조절 기반 암 제어 연구실
? 암 연구 및 치료제 개발은 유전자의 양적 발현변화 및 돌연변이에 의한 단백질 기능 변화에 집중되어 있지만 이러한 노력으로는 암에 관한 이해 및 극복 한계가 극명함. 따라서 본 연구를 통해 기존 암 연구의 미개척 영역인 암 특이적인 RNA 질적 대사 변화를 연구하고 비정상적인 암 유도 RNA 변화를 제어하는 antisense oligonucleotide ...
선택적 RNA 스플라이싱
RNA 생물학
RNA 기반 치료제
암
상보적 올리고뉴클레오타이드
최신 특허
특허 전체보기
전체 특허
칼슘을 이용한 세포 배양육의 제조방법 및 이에 따라 제조된 세포 배양육
상태
등록출원연도
2025출원번호
1020250105619PARP 억제제의 효능 예측용 PBRM1 유전자 마커 및 이의 용도
상태
등록출원연도
2025출원번호
1020250069148스플라이싱-스위치 올리고뉴클레오티드를 유효성분으로 포함하는 HTRA2 유전자의 스플라이싱 조절용 조성물
상태
등록출원연도
2025출원번호
1020250035139