CDy1, a PSC specific fluorescent probe, was investigated for the generation of reactive oxygen species (ROS) and demonstrated to induce selective death of PSC upon visible light irradiation. Importantly, the CDy1 and/or light irradiation did not negatively affect differentiated endothelial cells. The photodynamic treatment of PSC with CDy1 and visible light irradiation confirmed the inhibition of teratoma formation in mice.
ACS Cent. Sci., 2016
12
CO-1 and AzG-1, a cyclooctyne- and azide-containing BODIPY probe, respectively, can specifically label intracellular target organelles and engineered proteins with minimum background. They provide an efficient strategy for development of background-free probes, referred to as ‘tame’ probes, and novel tools for live cell intracellular imaging.
Nat. Commun., 2016
13
A multisite‐binding, switchable fluorescent probe can selectively and rapidly respond to ATP at intracellular concentrations. In live‐cell imaging, the probe, ATP-Red 1 was successfully applied to monitor fluctuations in mitochondrial ATP levels.
Angew. Chem. Int. Ed., 2016
14
CDy11 (compound of designation yellow 11), which targets amyloid in the Pseudomonas aeruginosa biofilm matrix, was discovered through a diversity-oriented fluorescent library approach (DOFLA). CDy11 was further demonstrated for in vivo imaging of P. aeruginosa in implant and corneal infection mice models.
J. Am. Chem. Soc., 2016
15
The oligomer-specific fluorescent chemical probe, BoDipy-Oligomer (BD-Oligo), was developed through the use of the diversity-oriented fluorescent library approach (DOFLA) and high-content, imaging-based screening. This probe enables dynamic oligomer monitoring during fibrillogenesis in vitro and shows in vivo Aβ oligomers staining possibility in the AD mice model.
J. Am. Chem. Soc., 2015
16
CDy9 is a highly reliable probe for the specific detection of live stem cells. CDy9 allows the detection and isolation of intact stem cells with marginal impact on their function and capabilities.
Bioorg. Med. Chem. Lett., 2015
17
A low-toxicity nucleus staining fluorescent probe, CDb12, was developed for real time mitosis imaging in live cells. CDb12 was identified by unbiased high-throughput imaging-based screening of a new xanthone library (AX). Unlike the conventional Hoechst dye, the low toxicity of CDb12 allows long term monitoring of cell division over more than one cell cycle.
Chem. Commun., 2015
18
CDy6 is a dye to mark mitotic cells. CDy6 displays numerous favorable properties including showing a sharp increase in intensity and change in localization in mitosis, improved photostability, and decreased toxicity compared with other widely used lysosomal markers in long-term real-time imaging.
Chem. Biol., 2015
19
To address existing limitations in live neuron imaging, we have developed NeuO, a novel cell-permeable fluorescent probe with an unprecedented ability to label and image live neurons selectively over other cells in the brain. NeuO enables robust live neuron imaging and isolation in vivo and in vitro across species; its versatility and ease of use sets the basis for its development in a myriad of neuronal targeting applications.
Angew. Chem. Int. Ed., 2015
20
The neural stem cell (NSC)-specific probe (CDr3), was developed through a high throughput/content screening of diversity-oriented fluorescence library in stem cells at different developmental stages. CDr3 specifically detects living neural stem cells of both human and mouse origin. Furthermore, the binding target was identified by proteomic analysis as fatty acid binding protein 7 (FABP7), which is highly expressed in neural stem cells and localized in the cytoplasm.