Mito thermo yellow was applied to measure mitochondrial temperature in situ under different physiological conditions. At a constant external temperature of 38 °C, mitochondria were more than 10 °C warmer (close to 50 °C) when the respiratory chain (RC) was fully functional, both in human embryonic kidney (HEK) 293 cells and primary skin fibroblasts.

ERthermAC was developed to monitor thermogenesis in BAs derived from murine brown fat precursors and in human brown fat cells differentiated from human neck brown preadipocytes. ERthermAC accumulated in the endoplasmic reticulum of BAs and displayed a marked change in fluorescence intensity in response to adrenergic stimulation of cells, which corresponded to temperature change.

ER thermo yellow is the first small molecule fluorescent thermometer selectively targeting the endoplasmic reticulum, with the highest sensitivity reported so far (3.9%/°C). Unlike nanoparticle thermometers, ER thermo yellow stains the target organelle evenly without the commonly encountered problem of aggregation, and successfully demonstrates the ability to monitor intracellular temperature gradients generated by external heat sources in various cell types. We further confirm the ability of ER thermo yellow to monitor heat production by intracellular Ca2+ changes in HeLa cells.

A simple BODIPY-based probe, BTV, was developed for cellular mitochondria viscosity imaging. BTV exhibited a significant fluorescence intensity enhancement as the solvent viscosity increased and showed a direct linear relationship between the fluorescence lifetime and the media viscosity. BTV could achieve practical applicability in the monitoring of mitochondrial viscosity changes in live cells through fluorescence lifetime imaging microscopy (FLIM).

Label-Free Enrichment of Highly Metastatic Tumor-Initiating Cells up to a Monoclonal State

Rewritable wavelength-selective hydrogel actuators grafted with fluorophores

Super-Photostable Organic Dye for Long-Term Live-cell Single Protein Imaging

A Non-Invasive, Label-Free Method for Examining Tardigrade Anatomy Using Holotomography

A Pair of Fluorescent Probes Enabling Precise Diagnosis of Liver Cancer by Complementary Imaging

Atom Efficient Synthesis of Trimethine Cyanines Using Formaldehyde as a Single Carbon Source