For the last two decades, Bacillus subtilis spore display system provided new platform for the various biotechnological applications, which overcame many obstacles of traditional bacterial surface display technology.Spore surface display needs Bacillus subtilis spore coat protein such as cotB, cotC and cotG for the anchoring of target protein.When the spore formation is completely done, mother-cell lyse, and spore is liberated to the environment.Because spore display system does not need secretory mechanism through bacterial cell wall or membrane, spore-based display technology has enhanced capability for the display of multimeric protein, large size protein, and co-factor associated protein which could disrupt cell membrane and cell wall of traditional bacterial surface display system.With spore's nascent robustness, spore surface display technology provides enhanced thermal stability, wide pH stability, organic solvent stability and repeated usage performance in whole cell enzyme reaction.Bacillus subtilis has been Generally Regarded as Safe (GRAS) for the traditional usage in soybean fermented food in Asia.This GRAS characteristics of Bacillus subtilis and its spore has been used for the development of spore based oral vaccine against human, feedstock cattle, fish, and worms.Spore display technology is widening its application in the fields of biosensor, environment, animal nutrition supplementation.In this paper, we describe recent development and application of spore surface technology and present new perspective for the near future.