A Chinese Hamster Transcription Start Site Atlas That Enables Targeted Editing of CHO Cells
I. Shamie, S. Duttke, K. Karottki, C. Han, A. Hansen, H. Hefzi, K. Xiong, S. Li, S. Roth, J. Tao, G. M. Lee, Glass, Christopher, H. Kildegaard, C. Benner, N.E. Lewis
NAR Genom. Bioinform., 2021
32
An Optimized Genome-Wide, Virus-Free CRISPR Screen for Mammalian Cells
K. Xiong, K. J. la Cour Karottki, H. Hefzi, Li S, L. M. Grav, S. Li, P. Spahn, J. S. Lee, G. M. Lee, H. F. Kildegaard, N. E. Lewis, L. E. Pedersen
Cell Rep. Methods, 2021
33
Blockage of Undesirable Endocytosis of Recombinant Human Growth/Differentiation Factor-5 in Chinese Hamster Ovary Cell Cultures Requires Heparin Analogs with Specific Chain Lengths
M. G. Kim, G. M. Lee
Biotechnol. J., 2021
34
Streamlined Human Cell-Based Recombinase-Mediated Cassette Exchange Platform Enables Multigene Expression for the Production of Therapeutic Proteins
S. Shin, S. H. Kim, J. S. Lee, G. M. Lee
ACS Synth. Biol., 2021
35
A Metabolic CRISPR-Cas9 Screen in Chinese Hamster Ovary Cells Identifies Glutamine-Sensitive Genes
K. J. la Cour Karottki, H. Hefzi, S. Li, L. E. Pedersen, P. N. Spahn, C. Joshi, D. Ruckerbauer, J. A. H. Bort, A. Thomas, J. S. Lee, N. Borth, G. M. Lee, H. F. Kildegaard, N. E. Lewis
Metabolic Eng., 2021
36
Selective Endocytosis Recombinant Human BMPs Through Cell Surface Heparan Sulfate Proteoglycans in CHO Cells: BMP-2 and BMP-7
M. G. Kim, C. L. Kim, Y. S. Kim, J. W. Jang, G. M. Lee
Sci. Rep., 2021
37
Comprehensive Characterization of Dihydrofolate Reductase-Mediated Gene Amplification for the Establishment of Recombinant Human Embryonic Kidney 293 Cells Producing Monoclonal Antibodies
S. Y. Lee, M. Baek, G. M. Lee
Biotechnol. J., 2021
38
Amplification of EBNA-1 through a single-plasmid vector-based gene amplification system in HEK293 cells as an efficient transient gene expression system
S. H. Park, J. H. Park, J. H. Lee, H. M. Lee, Y. J. Kang, E. J. Lee, S. Shin, G. M. Lee, Y. G. Kim
Appl. Microbiol. Biotechnol., 2020
39
Multi-Copy Targeted Integration for Accelerated Development of High-Producing CHO Cells
D. Sergeeva, L. K. Nielsen, G. M. Lee, L. M. Grav
ACS Synth. Biol., 2020
40
Forskolin Increases cAMP Levels and Enhances Recombinant Antibody Production in CHO Cell Cultures